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RAS PhysiologyРоссийский физиологический журнал им. И.М. Сеченова Russian Journal of Physiology

  • ISSN (Print) 0869-8139
  • ISSN (Online) 2658-655X

Effect of Ozone on the Oxygen-Transport Function of Blood and the Content of Gasotransmitters (Nitric Oxide and Hydrogen Sulfide) in Rats

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PII
S2658655X25090011-1
DOI
10.7868/S2658655X25090011
Publication type
Article
Status
Published
Authors
V.V. Zinchuk
  • Affiliation: Grodno State Medical University
M.A. Melenets
  • Affiliation: Grodno State Medical University
I.E. Gulyai
  • Affiliation: Grodno State Medical University
Volume/ Edition
Volume 111 / Issue number 9
Pages
1441-1452
Abstract
Ozone therapy is a highly effective method of rehabilitation of the body in various pathologies, the effects of which can be associated with its influence on the mechanisms of oxygen transport in the blood. The aim of this work is to study the effect of ozone in various concentrations on the oxygen-transport function of the blood, the content of 2,3-diphosphoglycerate and adenosine triphosphate, the gasotransmitter system (nitrogen monoxide and hydrogen sulfide) in rats. The experiment was performed on white outbred male rats ( = 56), which were divided into 4 groups: the control group, which received 1.0 ml of 0.9% NaCl solution intraperitoneally for 10 days, and 3 experimental groups, which were administered 0.9% NaCl solution with an ozone concentration of 1, 10 and 100 µg/kg of animal weight, respectively, for 10 days. Blood oxygen-transport function indices, 2,3-diphosphoglycerate and adenosine triphosphate, nitrate/nitrite and hydrogen sulfide content were determined. Animals that received ozone at a concentration of 10 µg/kg showed an increase in PO, SO, P50, 2,3-diphosphoglycerate, adenosine triphosphate and gas transmitters (nitrogen monoxide and hydrogen sulfide) compared to the control. No changes in blood oxygen-binding properties were observed when using ozone at minimum and maximum concentrations. The revealed effect of ozone (at a dose of 10 µg/kg) on rat blood oxygen-transport function, manifested in a decrease in hemoglobin affinity for oxygen, is realized through an increase in gas transmitters (nitrogen monoxide and hydrogen sulfide), promoting the growth of such modulators as 2,3-diphosphoglycerate and adenosine triphosphate.
Keywords
озон кровь кислород газотрансмиттер монооксид азота сероводород 2,3-дифосфоглицерат
Date of publication
21.12.2025
Year of publication
2025
Number of purchasers
0
Views
16

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