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RAS PhysiologyРоссийский физиологический журнал им. И.М. Сеченова Russian Journal of Physiology

  • ISSN (Print) 0869-8139
  • ISSN (Online) 2658-655X

Regulation of Intracellular Calcium during High-Frequency Rhythmic Stimulation of the Motor Nerve Ending of a Frog

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PII
S2658655X25090096-1
DOI
10.7868/S2658655X25090096
Publication type
Article
Status
Published
Authors
D. V. Samigullin
  • Affiliation:
    Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences
    Federal State Budgetary Educational Institution of Higher Education "Kazan National Research Technical University named after A.N. Tupolev – KAI"
N. F. Fatikhov
  • Affiliation: Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences
E. F. Khaziev
  • Affiliation: Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences
E. A. Bukharaeva
  • Affiliation: Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences
Volume/ Edition
Volume 111 / Issue number 9
Pages
1558-1575
Abstract
Under physiological conditions, chemical synapses, including neuromuscular junctions, operate rhythmically at different frequencies depending on the functional type of muscle and the state of synaptic contact. Calcium ions (Ca) entering the axoplasm through voltage-gated Ca channels during each action potential activate exocytosis of synaptic vesicles and play a key role in modulating the secretory process. The endoplasmic reticulum (ER), which can release Ca ions via Ca-dependent release, may contribute significantly to intracellular Ca dynamics. Optical recording techniques using Ca-sensitive fluorescent dyes are used to monitor changes in intracellular Ca. However, such an evaluation must pay special attention to the dye's binding characteristics with Ca ions, specifically its affinity, because the degree of dye saturation affects the parameters of the Ca response being investigated. In this study, the low-affinity dye Magnesium Green was used to analyze changes in the intracellular Ca ions concentration in the neuromuscular synapse m. cutaneus pectoris of the frog during rhythmic stimulation, which allows correct assessment of Ca signals. With increasing frequency of motor nerve stimulation, the smooth increase at 20 Hz of Ca response was replaced by a biphasic increase at 50 Hz and a sharp increase at 70 Hz. This indicates the inclusion of additional Ca sources, which may be the ER. Blocking ryanodine and inositol receptors abolished the increase in Ca response at higher frequencies of nerve stimulation. Blocking Ca ATPases ER (SERCA) resulted in a dramatic increase in the Ca response and eliminated its multiphasic character. It is shown that the change in Ca transient reflects the accumulation of intracellular Ca in the axoplasm and depends on the activity of SERCA, ryanodine and inositol receptors. The use of a low affinity fluorescent Ca dye makes it possible to track the contribution of these systems to the formation of the intracellular concentration of the main ions that determine the process of neurosecretion.
Keywords
ионы кальция нервно-мышечное соединение рианодиновые рецепторы инозительные рецепторы кальциевые ATФазы
Date of publication
21.12.2025
Year of publication
2025
Number of purchasers
0
Views
13

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